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Promoter Activation with Electromagnetism

  • Abraham O. Rodríguez-De la Fuente
  • J. Antonio Heredia-RojasEmail author
  • Juan M. Alcocer-González
  • Laura E. Rodríguez-Flores
  • Cristina Rodríguez-Padilla
  • Reyes S. Taméz-Guerra
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1651)

Abstract

Gene expression can be modified by physical factors, such as heat, electricity and magnetic fields, and several types of ionizing and non-ionizing radiation. Promoter activation with extremely low-frequency electromagnetic fields is possible with an appropriate promoter, containing electromagnetic field response elements. Here, we describe how to examine promoter activation with extremely low-frequency electromagnetic fields, and we provide a step-by-step guide to the assembly of a solenoid suitable for promoter activation.

Key words

Promoter activation Electromagnetic field Synthetic promoter Electromagnetic field response elements 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Abraham O. Rodríguez-De la Fuente
    • 1
  • J. Antonio Heredia-Rojas
    • 1
    Email author
  • Juan M. Alcocer-González
    • 1
  • Laura E. Rodríguez-Flores
    • 2
  • Cristina Rodríguez-Padilla
    • 1
  • Reyes S. Taméz-Guerra
    • 1
  1. 1.Universidad Autónoma de Nuevo LeónUANL, Facultad de Ciencias BiológicasSan Nicolás de los GarzaMexico
  2. 2.Universidad Autónoma de Nuevo León, UANLFacultad de MedicinaMonterreyMexico

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