A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation
It has been pointed out that ultrasound could be used as a controller for bioprocesses including gene expression since its energy can noninvasively reach deep in the body. Gene expression may be timely and spatially controlled by ultrasound, thus providing necessary bioactive proteins for the targeted tissue in a timely fashion. Although there are many processes involved in gene expression control, one of the most important processes is transcription, and the promoter plays an essential role in it. There are several promoters known to be activated in response to ultrasound irradiation. However, in our opinion, an artificial promoter is more suitable for clinical use. We herein describe simple methods to construct promoters that are responsive to ultrasound irradiation by randomly combining cis-elements (transcription factor binding motifs) and thereby improve its reactivity to ultrasound irradiation.
Key wordsUltrasound Oxidative stress TATA box Cis-element Directed evolution Error-prone PCR
This study was supported in part by Grants-in-Aid for Scientific Research (C) (21500403) and (18500374) and for Young Scientist (B) (20377253) and (23791746) from Japan Society for the Promotion of Science, the Research and Development Committee Program of the Japan Society of Ultrasonics in Medicine, a New Manufacture and Venture Creation Grant (Biotechnology) of Toyama New Industry Organization, a research grant of the First Bank of Toyama Foundation, and by Collaborative Research Project of the Wakasa Wan Energy Research Center.
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