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Avian and Reptilian Developmental Biology pp 191–202Cite as

Enhancer Analyses Using Chicken Embryo Electroporation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1650))

Abstract

Chicken embryo electroporation is a powerful tool used to identify and analyze enhancers involved in developmental gene regulation. In this chapter, the basic procedures and underlying principles of enhancer analysis using chicken embryo electroporation are described in the following steps: (1) identification of enhancers in a wide genomic region, (2) determination of the full enhancer region, (3) definition of the core enhancer regions, and (4) analysis of a functional transcription factor binding sequences in the core region.

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References

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Author information

Authors and Affiliations

  1. Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Masanori Uchikawa

  2. Shizuoka Prefecture Special Education Schools, Yahatano, Ito, Shizuoka, 413-0232, Japan

    Naoko Nishimura

  3. Smilow Center for Translational Research, University of Pennsylvania Perelman School of Medicine, 3400 Civic Center Blvd., Bldg. 421, Philadelphia, PA, 19104-5157, USA

    Makiko Iwafuchi-Doi

  4. Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto, 603-8555, Japan

    Hisato Kondoh

Authors
  1. Masanori Uchikawa
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  2. Naoko Nishimura
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  3. Makiko Iwafuchi-Doi
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  4. Hisato Kondoh
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Corresponding author

Correspondence to Hisato Kondoh .

Editor information

Editors and Affiliations

  1. IRCMS, Kumamoto University, Kumamoto, Kumamoto, Japan

    Guojun Sheng

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Uchikawa, M., Nishimura, N., Iwafuchi-Doi, M., Kondoh, H. (2017). Enhancer Analyses Using Chicken Embryo Electroporation. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7216-6_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7215-9

  • Online ISBN: 978-1-4939-7216-6

  • eBook Packages: Springer Protocols

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