Hemostasis and Thrombosis pp 369-389 | Cite as
Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood
Protocol
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Abstract
Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.
Key words
Platelet function Flow cytometry Whole blood Glycoprotein receptor deficiency Dense granule release Procoagulant platelets Calcium flux Platelet-leukocyte aggregatesReferences
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