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Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects In Vivo

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Fast Detection of DNA Damage

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1644))

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Abstract

In this chapter, we describe two new methodologies: (1) application of high-frequency ultrasound spectroscopy for in vivo detection of cancer cell death in small animal models, and (2) extension of ultrasound spectroscopy to the lower frequency range (i.e., 1–10 MHz range) for the detection of cell death in vivo in preclinical and clinical settings. Experiments using tumor xenografts in mice and cancer treatments based on chemotherapy are described. Finally, we describe how one can detect cancer response to treatment in patients noninvasively early (within 1 week of treatment initiation) using low-frequency ultrasound spectroscopic imaging and advanced machine learning techniques. Color-coded images of ultrasound spectroscopic parameters, or parametric images, permit the delineation of areas of dead cells versus viable cells using high ultrasound frequencies, and the delineation of areas of therapy response in patient tumors using clinically relevant ultrasound frequencies. Depending on the desired resolution, parametric ultrasound images can be computed and displayed within minutes to hours after ultrasound examination for cell death. A noninvasive and express method of cancer response detection using ultrasound spectroscopy provides a framework for personalized medicine with regards to the treatment planning of refractory patients resulting in substantial improvements in patient survival.

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Acknowledgments

M.J.G holds the Natural Sciences and Engineering Research Council of Canada Post-doctoral Fellowship. G.J.C. holds a University of Toronto James and Mary Davie Chair in Breast Cancer Imaging and Ablation. Funding for these projects was provided by the Terry Fox Foundation.

Author information

Authors and Affiliations

  1. Physical Sciences, Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, 2075 Bayview Avenue T2, Toronto, ON, Canada, M4N 3M5

    Hadi Tadayyon, Mehrdad J. Gangeh & Gregory J. Czarnota Ph.D., M.D.

  2. Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada, M4N 3M5

    Hadi Tadayyon, Mehrdad J. Gangeh & Gregory J. Czarnota Ph.D., M.D.

  3. Department of Radiation Oncology, Sunnybrook Health Sciences Centre, Toronto, ON, Canada, M4N 3M5

    Hadi Tadayyon, Mehrdad J. Gangeh & Gregory J. Czarnota Ph.D., M.D.

  4. Department of Radiation Oncology, Juravinski Cancer Centre, Hamilton Health Sciences, Hamilton, ON, Canada, L8V 5C2

    Roxana Vlad

  5. Department of Physics, Ryerson University, Toronto, ON, Canada, M5B 2K3

    Michael C. Kolios

  6. Department of Radiation Oncology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada, M5G 2M9

    Gregory J. Czarnota Ph.D., M.D.

Authors
  1. Hadi Tadayyon
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  2. Mehrdad J. Gangeh
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  3. Roxana Vlad
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  4. Michael C. Kolios
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  5. Gregory J. Czarnota Ph.D., M.D.
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Corresponding author

Correspondence to Gregory J. Czarnota Ph.D., M.D. .

Editor information

Editors and Affiliations

  1. Department of Neurosurgery and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

    Vladimir V. Didenko

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Tadayyon, H., Gangeh, M.J., Vlad, R., Kolios, M.C., Czarnota, G.J. (2017). Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects In Vivo. In: Didenko, V. (eds) Fast Detection of DNA Damage. Methods in Molecular Biology, vol 1644. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7187-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7187-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7185-5

  • Online ISBN: 978-1-4939-7187-9

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