Abstract
Site-specific recombinases are important tools for the modification of mammalian genomes. In conjunction with viral vectors, they can be utilized to mediate site-specific gene insertions in animals and in cell lines which are difficult to transfect. Here we describe a method for the generation and analysis of an adenovirus vector supporting a recombinase-mediated cassette exchange reaction and discuss the advantages and limitations of this approach.
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The original chapter was corrected: The affiliation of Dr. Andreas Kolb is University of Aberdeen, Foresterhill and not University of Foresterhill as stated originally in the EPUB version.
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Acknowledgments
This work was supported by the BBSRC (Gene Technologies underpinning Health Care #12599; CASE studentship BB/J012343/1), the Genomia Seed Fund, and the Hannah Development Fund.
The authors declare no conflicts of interest.
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Kolb, A.F. et al. (2017). Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors. In: Eroshenko, N. (eds) Site-Specific Recombinases. Methods in Molecular Biology, vol 1642. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7169-5_9
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DOI: https://doi.org/10.1007/978-1-4939-7169-5_9
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Publisher Name: Humana Press, New York, NY
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