Abstract
The technique of Cre-mediated DNA recombination at loxP sites has been used widely in manipulation of the genome in cultured cells and in living organisms. Local delivery of Cre recombinase protein tagged with a cell-penetrating (or permeable) peptide (Cre-CPP) has the advantage of additional spatial and temporal control when compared to genetic delivery methods. In this chapter, we describe protocols for injection-based intramuscular delivery of Cre-CPP dissolved in hydrogel to skeletal muscle and by ultrasound-guided injection to cardiac muscle in mice.
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Chien, WM., Liu, Y., Dinca, A.A., Chin, M.T. (2017). Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre. In: Eroshenko, N. (eds) Site-Specific Recombinases. Methods in Molecular Biology, vol 1642. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7169-5_7
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DOI: https://doi.org/10.1007/978-1-4939-7169-5_7
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7169-5
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