Abstract
Random insertional mutagenesis and subsequent reverse genetic screening allow the isolation of mutants of interest. Here I describe the protocol for generating a tag insertion line and subsequent PCR-based screening for ARGONAUTE mutants as an example of a reverse genetic screen for the unicellular green alga Chlamydomonas reinhardtii.
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References
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Yamasaki, T. (2017). Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas . In: Carbonell, A. (eds) Plant Argonaute Proteins. Methods in Molecular Biology, vol 1640. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7165-7_11
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DOI: https://doi.org/10.1007/978-1-4939-7165-7_11
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7165-7
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