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Characterization and Amplification of Gene-Based Simple Sequence Repeat (SSR) Markers in Date Palm

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Date Palm Biotechnology Protocols Volume II

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1638))

Abstract

The paucity of molecular markers limits the application of genetic and genomic research in date palm (Phoenix dactylifera L.). Availability of expressed sequence tag (EST) sequences in date palm may provide a good resource for developing gene-based markers. This study characterizes a substantial fraction of transcriptome sequences containing simple sequence repeats (SSRs) from the EST sequences in date palm. The EST sequences studied are mainly homologous to those of Elaeis guineensis and Musa acuminata. A total of 911 gene-based SSR markers, characterized with functional annotations, have provided a useful basis not only for discovering candidate genes and understanding genetic basis of traits of interest but also for developing genetic and genomic tools for molecular research in date palm, such as diversity study, quantitative trait locus (QTL) mapping, and molecular breeding. The procedures of DNA extraction, polymerase chain reaction (PCR) amplification of these gene-based SSR markers, and gel electrophoresis of PCR products are described in this chapter.

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References

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Author information

Authors and Affiliations

  1. Department of Agricultural and Environmental Science, College of Agriculture, Environment, and Nutrition Sciences, Tuskegee University, 1200 W. Montgomery Rd, Tuskegee, AL, 36088, USA

    Yongli Zhao & Guohao He

  2. National Clonal Germplasm Repository, USDA ARS, Riverside, CA, USA

    Manjunath Keremane

  3. College of Art and Science, Tuskegee University, Tuskegee, AL, USA

    Channapatna S. Prakash

Authors
  1. Yongli Zhao
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  2. Manjunath Keremane
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  3. Channapatna S. Prakash
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  4. Guohao He
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Corresponding author

Correspondence to Guohao He .

Editor information

Editors and Affiliations

  1. Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, Saudi Arabia

    Jameel M. Al-Khayri

  2. Agricultural Sciences, University of Helsinki, Helsinki, Finland

    S.Mohan Jain

  3. Cincinnati, Ohio, USA

    Dennis V. Johnson

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© 2017 Springer Science+Business Media LLC

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Zhao, Y., Keremane, M., Prakash, C.S., He, G. (2017). Characterization and Amplification of Gene-Based Simple Sequence Repeat (SSR) Markers in Date Palm. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7159-6_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7158-9

  • Online ISBN: 978-1-4939-7159-6

  • eBook Packages: Springer Protocols

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