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Temporary Immersion System for Date Palm Micropropagation

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Date Palm Biotechnology Protocols Volume I

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1637))

Abstract

The temporary immersion system (TIS) is being used with tremendous success for automation of micropropagation of many plant species. TIS usually consists of a culture vessel comprising two compartments, an upper one with the plant material and a lower one with the liquid culture medium and an automated air pump. The latter enables contact between all parts of the explants and the liquid medium by setting overpressure to the lower part of the container. These systems are providing the most satisfactory conditions for date palm regeneration via shoot organogenesis and allow a significant increase of multiplication rate (5.5-fold in comparison with that regenerated on agar-solidified medium) and plant material quality, thereby reducing production cost.

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Author information

Authors and Affiliations

  1. Laboratoire de Culture In Vitro, Centre Régional de Recherches en Agriculture Oasiennes, Degache, Tunisia

    Ahmed Othmani & Amel Sellemi

  2. Centre Régional de Recherches en Horticulture et Agriculture Biologique, Chott Mariem, Tunisie

    Chokri Bayoudh

  3. Laboratoire de Biotechnologie des Plantes, Faculté des Sciences, Université de Sfax, Sfax, Tunisie

    Noureddine Drira

Authors
  1. Ahmed Othmani
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  2. Chokri Bayoudh
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  3. Amel Sellemi
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  4. Noureddine Drira
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Corresponding author

Correspondence to Ahmed Othmani .

Editor information

Editors and Affiliations

  1. Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, Saudi Arabia

    Jameel M. Al-Khayri

  2. Agricultural Sciences, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  3. Cincinnati, Ohio, USA

    Dennis V. Johnson

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Othmani, A., Bayoudh, C., Sellemi, A., Drira, N. (2017). Temporary Immersion System for Date Palm Micropropagation. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_20

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  • DOI: https://doi.org/10.1007/978-1-4939-7156-5_20

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7155-8

  • Online ISBN: 978-1-4939-7156-5

  • eBook Packages: Springer Protocols

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