Abstract
Isolation by size using a filter membrane offers an antigen-independent method for capturing rare cells present in blood of cancer patients. Multiple cell types, including circulating tumor cells (CTCs), captured on the filter membrane can be simultaneously identified via immunocytochemistry (ICC) analysis of specific cellular biomarkers. Here, we describe an automated microfluidic filtration method combined with a liquid handling system for sequential ICC assays to detect and enumerate non-hematologic rare cells in blood.
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References
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Acknowledgments
We acknowledge Mary Foltz, Karen Marfurt, Cathleen Hanau Taylor, and other colleagues at Siemens Healthcare Diagnostic who helped prepare antibody, conjugates, and assay formats. We also acknowledge the clinical work done at the University of California-San Francisco by Jin Sun Lee, Marc Jabon, Victoria Wang, Matthew Gubens, and Hope S. Rugo. Mark Jesus M. Magbanua acknowledges the support received from the Breast Cancer Research Foundation.
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Pugia, M., Magbanua, M.J.M., Park, J.W. (2017). Automated Microfluidic Filtration and Immunocytochemistry Detection System for Capture and Enumeration of Circulating Tumor Cells and Other Rare Cell Populations in Blood. In: M. Magbanua, M., W. Park, J. (eds) Circulating Tumor Cells. Methods in Molecular Biology, vol 1634. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7144-2_9
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DOI: https://doi.org/10.1007/978-1-4939-7144-2_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7143-5
Online ISBN: 978-1-4939-7144-2
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