Separable Bilayer Microfiltration Device for Label-Free Enrichment of Viable Circulating Tumor Cells

  • Sijie Hao
  • Merisa Nisic
  • Hongzhang He
  • Yu-Chong TaiEmail author
  • Si-Yang ZhengEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1634)


Analysis of rare circulating tumor cells enriched from metastatic cancer patients yields critical information on disease progression, therapy response, and the mechanism of cancer metastasis. Here we describe in detail a label-free enrichment process of circulating tumor cells based on its unique physical properties (size and deformability). Viable circulating tumor cells can be successfully enriched and analyzed, or easily released for further characterization due to the novel separable two-layer design.

Key words

Circulating tumor cells Viable CTC isolation Cancer metastasis CTC in vitro culture Microfabrication 



S.-Y. Zheng thanks the Penn State Materials Research Institute, Nanofabrication laboratory and Microscopy and Cytometry facility, and Penn State Hershey Cancer Institute for their support. This work was partially supported by the Pennsylvania State University start-up fund and the National Cancer Institute of the National Institutes of Health under Award Number DP2CA174508.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Micro & Nano Integrated Biosystem (MINIBio) Laboratory, Department of Biomedical Engineering, Materials Research Institute, and Huck Life Science InstitutesThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Micro & Nano Integrated Biosystem (MINIBio) Laboratory, Department of Electrical Engineering, Materials Research Institute, and Huck Life Science InstitutesThe Pennsylvania State UniversityUniversity ParkUSA
  3. 3.Department of Electrical EngineeringCalifornia Institute of TechnologyPasadenaUSA
  4. 4.Department of Mechanical EngineeringCalifornia Institute of TechnologyPasadenaUSA
  5. 5.Department of Medical EngineeringCalifornia Institute of TechnologyPasadenaUSA
  6. 6.Division of Engineering and Applied ScienceCalifornia Institute of TechnologyPasadenaUSA

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