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Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells

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Circulating Tumor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1634))

Abstract

Circulating tumor cells (CTCs) may represent an easily accessible source of tumor material to assess genetic aberrations such as gene-rearrangements or gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs is a critical parameter to identify such biomarkers, we developed fluorescent in situ hybridization (FISH) for CTCs enriched on filters (filter-adapted-FISH, FA-FISH). Here, we describe the FA-FISH protocol, the combination of immunofluorescent staining (DAPI/CD45) and FA-FISH techniques, as well as the semi-automated microscopy method that we developed to improve the feasibility and reliability of FISH analyses in filtration-enriched CTC.

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Correspondence to Françoise Farace .

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Oulhen, M., Pailler, E., Faugeroux, V., Farace, F. (2017). Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells. In: M. Magbanua, M., W. Park, J. (eds) Circulating Tumor Cells. Methods in Molecular Biology, vol 1634. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7144-2_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7144-2_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7143-5

  • Online ISBN: 978-1-4939-7144-2

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