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Circulating Tumor Cells pp 133–141Cite as

Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells

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Part of the Methods in Molecular Biology book series (MIMB,volume 1634)

Abstract

Circulating tumor cells (CTCs) may represent an easily accessible source of tumor material to assess genetic aberrations such as gene-rearrangements or gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs is a critical parameter to identify such biomarkers, we developed fluorescent in situ hybridization (FISH) for CTCs enriched on filters (filter-adapted-FISH, FA-FISH). Here, we describe the FA-FISH protocol, the combination of immunofluorescent staining (DAPI/CD45) and FA-FISH techniques, as well as the semi-automated microscopy method that we developed to improve the feasibility and reliability of FISH analyses in filtration-enriched CTC.

Key words

  • Circulating tumor cells
  • FISH
  • FA-FISH
  • ISET
  • Filtration-enrichment

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  • DOI: 10.1007/978-1-4939-7144-2_10
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Author notes

  1. $Marianne Oulhen and Emma Pailler contributed equally to this work.

Authors and Affiliations

  1. Gustave Roussy, AMMICA CNRS U MS3655 – INSERM US23, Université Paris-Saclay, 94805, Villejuif, France

    Marianne Oulhen, Emma Pailler, Vincent Faugeroux & Françoise Farace

  2. INSERM, U981, Gustave Roussy, Université Paris-Saclay, 114 rue Édouard Vaillant, 94805, Villejuif, France

    Marianne Oulhen, Emma Pailler, Vincent Faugeroux & Françoise Farace

  3. University of Paris-Sud, Université Paris-Saclay, 94270, Le Kremlin-Bicetre, France

    Emma Pailler, Vincent Faugeroux & Françoise Farace

Authors
  1. Marianne Oulhen
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  2. Emma Pailler
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  3. Vincent Faugeroux
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  4. Françoise Farace
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Corresponding author

Correspondence to Françoise Farace .

Editor information

Editors and Affiliations

  1. Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer Center, University of California-San Francisco, San Francisco, California, USA

    Mark Jesus M. Magbanua

  2. Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer Center, University of California-San Francisco, San Francisco, California, USA

    John W. Park

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Oulhen, M., Pailler, E., Faugeroux, V., Farace, F. (2017). Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells. In: M. Magbanua, M., W. Park, J. (eds) Circulating Tumor Cells. Methods in Molecular Biology, vol 1634. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7144-2_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7144-2_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7143-5

  • Online ISBN: 978-1-4939-7144-2

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