Comprehensive Shotgun Proteomic Analyses of Oligodendrocytes Using Ion Mobility and Data-Independent Acquisition

Protocol
Part of the Neuromethods book series (NM, volume 127)

Abstract

Oligodendrocytes are a type of neuroglia that provide trophic support and axonal insulation of the central nervous system. A proliferating clonal oligodendrocyte cell line, named MO3.13, has been developed to enable the comprehension of the biological role of these cells in the central nervous system in a controled environment. In the present protocol, we established a comprehensive proteomic characterization of MO3.13 cells using 2D LC fractionation and ion mobility-enhanced data-independent MS analyses. The final dataset of identified proteins may consist a rich source of molecular information about oligodendrocytes. Also, it can help further studies using MO3.13 cells as a tool of investigation not only to oligodendrocyte maturation but also to diseases that have oligodendrocytes as key players.

Key words

Oligodendrocytes Proteome Data-independent analysis Ion mobility separation 2D LC fractionation 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Laboratory of Neuroproteomics, Department of Biochemistry and Tissue BiologyInstitute of Biology, University of Campinas (UNICAMP)CampinasBrazil

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