Extracellular vesicles (EVs) play a key role in cell-to-cell communication carrying lipids, genetic material, and proteins, producing changes in their target cells. In the last years, EVs have been a hot topic owning to their potential source of biomarkers in many diseases. The search for new biomarkers of central nervous diseases is limited due to the difficulty in accessing to biopsies or complications after obtaining cerebrospinal fluid. In this sense, the study of EVs obtained from blood is proposed as a source of surrogate biomarkers of easy access. One of the most studied components of EVs as biomarkers is their protein cargo. In this chapter we present the main protocols for EV isolation from blood and urine samples, these being based on differential centrifugation and commercial kits for polymeric precipitation. Subsequently, the protocol for a proteomic characterization of plasma-derived EVs is also detailed.
Extracellular vesicle Isolation protocol Proteomics Mass spectrometry
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1.Immunology UnitDonostia University HospitalSan SebastiánSpain
2.Multiple Sclerosis GroupBiodonostia Health Research InstituteDonostia-San SebastiánSpain
3.Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health DepartmentPublic University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA)PamplonaSpain