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Beta-Glucan Particles as Vaccine Adjuvant Carriers

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Vaccines for Invasive Fungal Infections

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1625))

Abstract

Glucan particles (GPs) are spherical hollow particles derived from Saccharomyces cerevisiae cell walls and mainly consist of β-1, 3-d-glucans. The inner hollow cavity of glucan particles can be loaded with different compounds, including protein antigens, and delivered to macrophages and dendritic cells. Moreover, the GP delivery system possesses β-glucan’s intrinsic immunostimulatory properties. Therefore, GPs serve as both an antigen-presenting cell-targeted delivery system and an adjuvant.

Here, we describe the production of GPs from S. cerevisiae using hot alkaline and solvent extraction and characterization of these particles for morphology, particle density, and hydrodynamic volume. A detailed protocol for loading and entrapping a model antigen, ovalbumin (OVA), into these particles using yeast RNA is presented. Similar methods are used to load pathogen-specific antigens (peptides, proteins, soluble extracts) which then can be tested in in vivo vaccination models.

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Author information

Authors and Affiliations

  1. Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USA

    Zeynep Mirza, Ernesto R. Soto & Fusun Dikengil

  2. Department of Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USA

    Stuart M. Levitz

  3. Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation St., Suite 113, Worcester, MA, 01605, USA

    Gary R. Ostroff

Authors
  1. Zeynep Mirza
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  2. Ernesto R. Soto
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  3. Fusun Dikengil
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  4. Stuart M. Levitz
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  5. Gary R. Ostroff
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Corresponding author

Correspondence to Gary R. Ostroff .

Editor information

Editors and Affiliations

  1. Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, California, USA

    Markus Kalkum

  2. Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, California, USA

    Margarita Semis

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Mirza, Z., Soto, E.R., Dikengil, F., Levitz, S.M., Ostroff, G.R. (2017). Beta-Glucan Particles as Vaccine Adjuvant Carriers. In: Kalkum, M., Semis, M. (eds) Vaccines for Invasive Fungal Infections. Methods in Molecular Biology, vol 1625. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7104-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7104-6_11

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7103-9

  • Online ISBN: 978-1-4939-7104-6

  • eBook Packages: Springer Protocols

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