Generation and Analysis of Chromosomal Contact Maps of Bacteria

  • Martial MarboutyEmail author
  • Romain KoszulEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1624)


This methods article described a protocol aiming at generating chromosome contact maps of bacterial species using a genome-wide derivative of the chromosome conformation capture (3C) technique. The approach is readily applicable on a broad variety of gram + and gram-bacterial species. It describes and addresses known caveats and technicalities associated with the technique, and should be of interest to any laboratory interested to perform a multiscale analysis of the genome structure of its species of interest.

Key words

Chromosome conformation capture, Hi-C 3C Genome organization Nucleoid 



This research was supported by funding to R.K. from the European Research Council under the seventh Framework Program (FP7/2007-2013, ERC grant agreement 260822) and from Agence Nationale pour la Recherche (HiResBaCS ANR-15-CE11-0023).


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Groupe Régulation Spatiale des Génomes, Département Génomes et GénétiqueInstitut PasteurParis Cedex 15France
  2. 2.CNRS, UMR 3525Paris Cedex 15France
  3. 3.Centre de Bioinformatique, Biostatistique et Biologie IntégrativeUSR 3756, Institut PasteurParis Cedex 15France

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