Abstract
In germinal centers (GCs), B cells undergo repeated cycles of proliferation and affinity-based selection, and differentiate into memory B cells or long-lived plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly because experimental manipulation of GC B cells has been limited. Here we describe a culture system in which we can induce massive expansion of naive B cells that exhibit GC B cell-like phenotype and acquire abilities to differentiate into memory B cells or bone marrow plasma cells depending on cytokine conditions. This system will allow us to elucidate the molecular mechanisms of GC B cell differentiation.
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Acknowledgment
This work was supported by Grant-in-Aid for Scientific Research (B) to D.K. The authors declare no financial conflict of interest.
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Haniuda, K., Nojima, T., Kitamura, D. (2017). In Vitro-Induced Germinal Center B Cell Culture System. In: Calado, D. (eds) Germinal Centers. Methods in Molecular Biology, vol 1623. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7095-7_11
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DOI: https://doi.org/10.1007/978-1-4939-7095-7_11
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