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DESI Mass Spectrometry Imaging (MSI)

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Imaging Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1618))

Abstract

Desorption Electrospray Ionization (DESI) mass spectrometry is a technique that allows chemical information to be obtained directly from a wide range of surfaces. Using a 2D stage, DESI can be implemented in an imaging mode whereby MS spectra are collected by rastering the spray across the whole surface. Here, we describe the implementation and optimization of DESI imaging for metabolites and lipids from tissue sections using oa-TOF mass spectrometers.

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Authors and Affiliations

  1. Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow, SK9 4AX, UK

    Emmanuelle Claude, Emrys A. Jones & Steven D. Pringle

Authors
  1. Emmanuelle Claude
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  2. Emrys A. Jones
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  3. Steven D. Pringle
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Corresponding author

Correspondence to Emmanuelle Claude .

Editor information

Editors and Affiliations

  1. The Center for Mass Spectrometry Imaging Biomolecular Science Research Center, Sheffield Hallam University, Sheffield, South Yorkshire, United Kingdom

    Laura M. Cole

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© 2017 Springer Science+Business Media LLC

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Claude, E., Jones, E.A., Pringle, S.D. (2017). DESI Mass Spectrometry Imaging (MSI). In: Cole, L. (eds) Imaging Mass Spectrometry . Methods in Molecular Biology, vol 1618. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7051-3_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7051-3_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7050-6

  • Online ISBN: 978-1-4939-7051-3

  • eBook Packages: Springer Protocols

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