Abstract
Fine needle aspiration (FNA) biopsies are the current gold-standard for the preoperative evaluation of thyroid nodules. However, a significant number of them (15–30%) are unable to be affirmatively diagnosed and are given an “indeterminate for malignancy” final report, meaning that the malignant nature of the thyroid nodule remains unknown and the recommended therapeutic approach is total thyroidectomy. Furthermore, cytomorphological evaluation of biopsies taken post-surgery indicates that approximately 80% of nodules within this group of patients are in fact benign, and the total thyroidectomy unwarranted. Therefore, the identification of new possible diagnostic targets that can assist in the preoperative diagnosis of thyroid tumors and reduce the number of unnecessary thyroidectomies is imperative.
Matrix-Assisted Laser Desorption/Ionization (MALDI)—Mass Spectrometry Imaging (MSI) has the ability to provide very precise and localized information regarding protein expression in cytological specimens. This enables the detection of cell subpopulations based on their different protein profiles, even within regions that are indistinguishable at the microscopic level, and the feasibility of this approach to investigate FNA specimens has already been highlighted in a number of studies. Here, an overview about the sample preparation procedure for the MALDI-MSI analysis of ex vivo FNA biopsies is provided, highlighting how molecular imaging can be combined with traditional histology to generate protein signatures of the different thyroid lesions, and, ultimately, build classification models that can be potentially used to classify benign and malignant thyroid nodules from a molecular standpoint.
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References
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D (2011) Global cancer statistics. CA Cancer J Clin 61(2):69–90
Cancer Statistics Review, 1975–2011—SEER Statistics [Internet]. Available from: http://seer.cancer.gov/csr/1975_2011/results_merged/sect_26_thyroid.pdf
Patel KN, Shaha AR (2014) Poorly differentiated thyroid cancer. Curr Opin Otolaryngol Head Neck Surg 22(2):121–126
Tzen C-Y, Huang Y-W, Fu Y-S (2003) Is atypical follicular adenoma of the thyroid a preinvasive malignancy? Hum Pathol 34(7):666–669
Vasko VV, Gaudart J, Allasia C et al (2004) Thyroid follicular adenomas may display features of follicular carcinoma and follicular variant of papillary carcinoma. Eur J Endocrinol Eur Fed Endocr Soc 151(6):779–786
American Thyroid Association (ATA) Guidelines Taskforce on Thyroid Nodules and Differentiated Thyroid Cancer, Cooper DS, Doherty GM et al (2009) Revised American Thyroid Association management guidelines for patients with thyroid nodules and differentiated thyroid cancer. Thyroid Off J Am Thyroid Assoc 19(11):1167–1214
La Vecchia C, Malvezzi M, Bosetti C et al (2015) Thyroid cancer mortality and incidence: a global overview. Int J Cancer J Int Cancer 136(9):2187–2195
Polyzos SA, Anastasilakis AD (2009) Clinical complications following thyroid fine-needle biopsy: a systematic review. Clin Endocrinol (Oxf) 71(2):157–165
Mainini V, Pagni F, Garancini M et al (2013) An alternative approach in endocrine pathology research: MALDI-IMS in papillary thyroid carcinoma. Endocr Pathol 24(4):250–253
Pagni F, Mainini V, Garancini M, et al (2015) Proteomics for the diagnosis of thyroid lesions: preliminary report. Cytopathol Off J Br Soc Clin Cytol 26(5):318–324
Pagni F, De Sio G, Garacini M et al (2016) Proteomics in thyroid cytopathology: relevance of MALDI-imaging in distinguishing malignant from benign lesions. Proteomics 16(11–12):1775–1784
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Mosele, N., Smith, A., Galli, M., Pagni, F., Magni, F. (2017). MALDI-MSI Analysis of Cytological Smears: The Study of Thyroid Cancer. In: Cole, L. (eds) Imaging Mass Spectrometry . Methods in Molecular Biology, vol 1618. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7051-3_5
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DOI: https://doi.org/10.1007/978-1-4939-7051-3_5
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