Abstract
The advances in molecular biology of the last decades have dramatically improved the field of diagnostic bacteriology. In particular, PCR-based technologies have impacted the diagnosis of infections caused by obligate intracellular bacteria such as pathogens from the Chlamydiacae family. Here, we describe a real-time PCR-based method using the Taqman technology for the diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus infection. The method presented here can be applied to various clinical samples and can be adapted on opened molecular diagnostic platforms.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lamoth F, Greub G (2010) Fastidious intracellular bacteria as causal agents of community-acquired pneumonia. Expert Rev Anti-Infect Ther 8(7):775–790. doi:10.1586/eri.10.52
Longbottom D, Coulter LJ (2003) Animal chlamydioses and zoonotic implications. J Comp Pathol 128(4):217–244
Yin L, Kalmar ID, Lagae S, Vandendriessche S, Vanderhaeghen W, Butaye P, Cox E, Vanrompay D (2013) Emerging Chlamydia psittaci infections in the chicken industry and pathology of Chlamydia psittaci genotype B and D strains in specific pathogen free chickens. Vet Microbiol 162(2–4):740–749. doi:10.1016/j.vetmic.2012.09.026
Magnino S, Haag-Wackernagel D, Geigenfeind I, Helmecke S, Dovc A, Prukner-Radovcic E, Residbegovic E, Ilieski V, Laroucau K, Donati M, Martinov S, Kaleta EF (2009) Chlamydial infections in feral pigeons in Europe: review of data and focus on public health implications. Vet Microbiol 135(1–2):54–67. doi:10.1016/j.vetmic.2008.09.045
Geigenfeind I, Vanrompay D, Haag-Wackernagel D (2012) Prevalence of Chlamydia psittaci in the feral pigeon population of Basel, Switzerland. J Med Microbiol 61(Pt 2):261–265. doi:10.1099/jmm.0.034025-0
Reinhold P, Ostermann C, Liebler-Tenorio E, Berndt A, Vogel A, Lambertz J, Rothe M, Ruttger A, Schubert E, Sachse K (2012) A bovine model of respiratory Chlamydia psittaci infection: challenge dose titration. PLoS One 7(1):e30125. doi:10.1371/journal.pone.0030125
Ostermann C, Ruttger A, Schubert E, Schrodl W, Sachse K, Reinhold P (2013) Infection, disease, and transmission dynamics in calves after experimental and natural challenge with a bovine Chlamydia psittaci isolate. PLoS One 8(5):e64066. doi:10.1371/journal.pone.0064066
Senn L, Greub G (2008) Local newspaper as a diagnostic aid for psittacosis: a case report. Clin Infect Dis 46(12):1931–1932. doi:10.1086/588562
Asner SA, Jaton K, Kyprianidou S, Nowak AM, Greub G (2014) Chlamydia pneumoniae: possible association with asthma in children. Clin Infect Dis 58(8):1198–1199. doi:10.1093/cid/ciu034
Greub G, Sahli R, Brouillet R, Jaton K (2016) Ten years of R&D and full automation in molecular diagnosis. Future Microbiol 11:403–425. doi:10.2217/fmb.15.152
Welti M, Jaton K, Altwegg M, Sahli R, Wenger A, Bille J (2003) Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions. Diagn Microbiol Infect Dis 45(2):85–95
Opota O, Jaton K, Branley J, Vanrompay D, Erard V, Borel N, Longbottom D, Greub G (2015) Improving the molecular diagnosis of Chlamydia psittaci and Chlamydia abortus infection with a species-specific duplex real-time PCR. J Med Microbiol (2015), 64, 1174–1185. doi:10.1099/jmm.0.000139
Jaton K, Brouillet R (2011) Full automation of nucleic acid extraction with the dual starlet (hamilton)-magnapure 96 (roche) system: a preliminary experience. ECCMID 2011, Milano
Acknowledgments
We are grateful to all the technicians of the Diagnostic Microbiology Laboratory of the Institute of Microbiology of the Lausanne University Hospital for their technical contribution.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Opota, O., Brouillet, R., Greub, G., Jaton, K. (2017). Methods for Real-Time PCR-Based Diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus Infections in an Opened Molecular Diagnostic Platform. In: Bishop-Lilly, K. (eds) Diagnostic Bacteriology. Methods in Molecular Biology, vol 1616. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7037-7_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7037-7_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7035-3
Online ISBN: 978-1-4939-7037-7
eBook Packages: Springer Protocols