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Bacterial Protein Secretion Systems pp 87–95Cite as

Cell Surface Exposure

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1615))

Abstract

Surface-exposed proteins of Gram-negative bacteria are represented by integral outer membrane beta-barrel proteins and lipoproteins. No computational methods exist for predicting surface-exposed lipoproteins, and therefore lipoprotein topology must be experimentally tested. This chapter describes three distinct but complementary methods for the detection of surface-exposed proteins: cell surface protein labeling, accessibility to extracellular protease and antibodies.

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Authors and Affiliations

  1. Lewis Thomas Laboratory, Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ, 08544, USA

    Anna Konovalova

Authors
  1. Anna Konovalova
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Correspondence to Anna Konovalova .

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Editors and Affiliations

  1. Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    Laure Journet

  2. Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    Eric Cascales

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Konovalova, A. (2017). Cell Surface Exposure. In: Journet, L., Cascales, E. (eds) Bacterial Protein Secretion Systems. Methods in Molecular Biology, vol 1615. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7033-9_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7033-9_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7031-5

  • Online ISBN: 978-1-4939-7033-9

  • eBook Packages: Springer Protocols

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