Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta

  • Yu Wang
  • Lisa A. Cassis
  • Sean E. ThatcherEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1614)


The use of fluorogenic substrates to measure enzymatic activity is widely used to understand function within different experimental models. ACE2 is important in understanding the balance between AngII and Ang-(1-7) and how this balance could then in turn influence hypertension or other disease outcomes. Here, we describe a method to measure ACE2 activity in abdominal aorta of hyperlipidemic mice under both saline and AngII infusion.

Key words

Angiotensin converting enzyme 2 Aorta Activity Enzyme Fluorescence 


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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Pharmacology and Nutritional SciencesUniversity of KentuckyLexingtonUSA
  2. 2.Department of Pharmacology and Nutritional SciencesUniversity of KentuckyLexingtonUSA

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