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3D Cell Culture pp 391–398Cite as

Bioprinting Cartilage Tissue from Mesenchymal Stem Cells and PEG Hydrogel

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1612))

Abstract

Bioprinting based on thermal inkjet printing is one of the most attractive enabling technologies for tissue engineering and regeneration. During the printing process, cells, scaffolds , and growth factors are rapidly deposited to the desired two-dimensional (2D) and three-dimensional (3D) locations. Ideally, the bioprinted tissues are able to mimic the native anatomic structures in order to restore the biological functions. In this study, a bioprinting platform for 3D cartilage tissue engineering was developed using a commercially available thermal inkjet printer with simultaneous photopolymerization . The engineered cartilage demonstrated native zonal organization, ideal extracellular matrix (ECM ) composition, and proper mechanical properties. Compared to the conventional tissue fabrication approach, which requires extended UV exposure, the viability of the printed cells with simultaneous photopolymerization was significantly higher. Printed neocartilage demonstrated excellent glycosaminoglycan (GAG) and collagen type II production, which was consistent with gene expression profile. Therefore, this platform is ideal for anatomic tissue engineering with accurate cell distribution and arrangement.

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References

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Acknowledgments

This work was supported by the Fundamental Research Funds for the Central Universities (WUT: 2015IB004, 2017IB004).

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Authors and Affiliations

  1. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 122 Luoshi Rd., Wuhan, 430070, China

    Guifang Gao & Xiaofeng Cui Ph.D.

  2. Stemorgan Therapeutics, 90 State St., Albany, NY, 12207, USA

    Guifang Gao, Karen Hubbell & Xiaofeng Cui Ph.D.

  3. Clinic for Plastic Surgery and Hand Surgery, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany

    Arndt F. Schilling & Xiaofeng Cui Ph.D.

  4. Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th St., Troy, NY, 12180, USA

    Guohao Dai & Xiaofeng Cui Ph.D.

Authors
  1. Guifang Gao
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  2. Karen Hubbell
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  3. Arndt F. Schilling
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  4. Guohao Dai
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  5. Xiaofeng Cui Ph.D.
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Corresponding author

Correspondence to Xiaofeng Cui Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Histology and Embryology, Masaryk University, Brno, Czech Republic

    Zuzana Koledova

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Gao, G., Hubbell, K., Schilling, A.F., Dai, G., Cui, X. (2017). Bioprinting Cartilage Tissue from Mesenchymal Stem Cells and PEG Hydrogel. In: Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 1612. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7021-6_28

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  • DOI: https://doi.org/10.1007/978-1-4939-7021-6_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7019-3

  • Online ISBN: 978-1-4939-7021-6

  • eBook Packages: Springer Protocols

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