Abstract
Three-dimensional (3D) tumor culture miniaturized platforms are of importance to biomimetic model construction and pathophysiological studies. Controllable and high-throughput production of 3D tumors is desirable to make cell-based manipulation dynamic and efficient at micro-scale. Moreover, the 3D culture platform being reusable is convenient to research scholars. In this chapter, we describe a dynamically controlled 3D tumor manipulation and culture method using pneumatic microstructure-based microfluidics, which has potential applications in the fields of tissue engineering, tumor biology, and clinical medicine in a high-throughput way.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31470971, No. 21375106, No. 21175107, No. 31100726), the program of China Scholarships Council (No. 201208610047), the Fundamental Research Funds for the Central Universities (No. 2452015439), and Northwest A&F University.
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Liu, W., Wang, J. (2017). High-Throughput 3D Tumor Culture in a Recyclable Microfluidic Platform. In: Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 1612. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7021-6_22
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DOI: https://doi.org/10.1007/978-1-4939-7021-6_22
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