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3D Cell Culture: An Introduction

Part of the Methods in Molecular Biology book series (MIMB,volume 1612)

Abstract

3D cell culture is an invaluable tool in developmental, cell, and cancer biology. By mimicking crucial features of in vivo environment, including cell–cell and cell–extracellular matrix interactions, 3D cell culture enables proper structural architecture and differentiated function of normal tissues or tumors in vitro. Thereby 3D cell culture realistically models in vivo tissue conditions and processes, and provides in vivo like responses. Since its early days in the 1970s, 3D cell culture has revealed important insights into mechanisms of tissue homeostasis and cancer, and accelerated translational research in cancer biology and tissue engineering.

Key words

  • 3D cell culture
  • Bioprinting
  • Extracellular matrix
  • Microenvironment
  • Microfluidics
  • Spheroid
  • Tissue architecture

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  • DOI: 10.1007/978-1-4939-7021-6_1
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Acknowledgments

This work was supported by the project “Employment of Best Young Scientists for International Cooperation Empowerment” (CZ.1.07/2.3.00/30.0037) cofinanced from European Social Fund and the state budget of the Czech Republic, by the grant “Junior investigator 2015” (Faculty of Medicine, Masaryk University), and by the grant 16-20031Y from Czech Science Foundation (GACR).

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Correspondence to Zuzana Koledova .

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Koledova, Z. (2017). 3D Cell Culture: An Introduction. In: Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 1612. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7021-6_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7021-6_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7019-3

  • Online ISBN: 978-1-4939-7021-6

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