Abstract
Three-dimensional (3D) cell culture enables the growth of cells in a multidimensional and multicellular manner compared to conventional cell culture techniques. Especially in prostate cancer research there is a big need for more tissue-recapitulating models to get a better understanding of the mechanisms driving prostate cancer as well as to screen for more efficient drugs that can be used for treatment. In this chapter we describe a 3D hanging drop system that can be used to culture prostate cancer organoids as tumor epithelial monocultures and as epithelial–stromal cocultures.
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Acknowledgments
This work was supported by a grant from the Medizinischer Forschungsfonds (MFF), Innsbruck, Austria, and a research grant from Astellas Pharma Ges.m.b.H. We thank Irma Sottsas and Georg Grünbacher for technical assistance and Dr. Christian Ploner and Dr. Gabriele Gamerith for helpful hints regarding 3D cell culture. We also thank Prof. Helmut Klocker for reading the manuscript.
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Eder, T., Eder, I.E. (2017). 3D Hanging Drop Culture to Establish Prostate Cancer Organoids. In: Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 1612. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7021-6_12
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DOI: https://doi.org/10.1007/978-1-4939-7021-6_12
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