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Applications of X-Ray Micro-Beam for Data Collection

  • Ruslan Sanishvili
  • Robert F. Fischetti
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1607)

Abstract

Micro-diffraction tools for macromolecular crystallography, first developed at the end of 1990s and now an integral part of many synchrotron beamlines, enable some of the experiments which were not feasible just a decade or so ago. These include data collection from very small samples, just a few micrometers in size; from larger, but severely inhomogeneous samples; and from samples which are optically invisible. Improved micro-diffraction tools led to improved signal-to-noise ratio, to mitigation of radiation damage in some cases, and to better-designed diffraction experiments. Small, micron-scale beams can be attained in different ways and knowing the details of the implementation is important in order to design the diffraction experiment properly. Similarly, precision, reproducibility and stability of the goniometry, and caveats of detection systems need to be taken into account. Lastly, to make micro-diffraction widely applicable, the sophistication, robustness, and user-friendliness of these tools are just as important as the technical capabilities.

Key words

Micro-beam Micro-diffraction Micro-focus Raster Small crystals Inhomogeneous crystals Signal-to-noise Radiation damage Multi-crystal data collection 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.GM/CA@APS, Advanced Photon SourceArgonne National LaboratoryArgonneUSA

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