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Protein Crystallography pp 325–347Cite as

Processing of XFEL Data

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1607))

Abstract

The introduction of the X-ray laser to crystallography, and its impact on the types of crystallographic experiments being performed as described in the previous chapter, has meant that new data processing strategies had to be found. While some XFEL crystallography experiments approach the conventional methods quite closely, even those are not without special considerations relating to data processing. Serial femtosecond crystallography (SFX) introduces several additional problems, many of which have been solved recently, and there has been great progress towards resolving the remaining ones. Recent developments into the use of continuous scattering between the Bragg peaks will need even greater changes to the conventional data processing methods. This chapter describes the special characteristics of XFEL data and introduces the range of processing methods which are currently under development.

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Acknowledgments

The author acknowledges financial support from the Helmholtz Association through programme oriented funds, and thanks Anton Barty and Nadia Zatsepin for reading and providing helpful suggestions on the manuscript.

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Authors and Affiliations

  1. Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607, Hamburg, Germany

    Thomas A. White

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  1. Thomas A. White
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Correspondence to Thomas A. White .

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Editors and Affiliations

  1. Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland, USA

    Alexander Wlodawer

  2. Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, Illinois, USA

    Zbigniew Dauter

  3. Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland

    Mariusz Jaskolski

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White, T.A. (2017). Processing of XFEL Data. In: Wlodawer, A., Dauter, Z., Jaskolski, M. (eds) Protein Crystallography. Methods in Molecular Biology, vol 1607. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7000-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7000-1_13

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