On Electrochemical Methods for Determination of Protein-Lipid Interaction
Amyloid-β (Aβ) peptides are important and reliable molecular biomarkers for the diagnosis and prognosis of Alzheimer’s disease. Aggregation and fibrillization of Aβ peptides on ganglioside GM1 (GM1)-containing lipid membranes is considered a cause of neurodegenerative disease. Because GM1 is abundant in the central nervous system and plays a key role in the aggregation of Aβ, the interaction of Aβ with supported planar lipid bilayers (SPBs) containing GM1 is of great significance. We have prepared SPBs containing GM1 in order to study the electrochemical characteristics of GM1/sphingomyelin/cholesterol SPBs and their interaction with Aβ(1–40) by cyclic voltammetry and electrochemical impedance spectroscopy (EIS), which proves that electrochemical is a promising method for analyzing the interaction between peptides and lipid membranes.
Key wordsProtein-lipid interaction Electrochemical Alzheimer’s disease Amyloid-β Ganglioside GM1 Lipid bilayer
This work was supported by National Natural Science Foundation of China (Grant No. 21103043), the Science and technology research project of Henan province (No. 142102210389), the National Science Foundation of China (No. 21173068), and the Program for Innovative Research Team (in Science and Technology) in the University of Henan Province (No. 13IRTSTHN01).