Skip to main content

Entry Studies of New World Arenaviruses

  • 1672 Accesses

Part of the Methods in Molecular Biology book series (MIMB,volume 1604)

Abstract

Identification of cell moieties involved in viral binding and internalization is essential since their expression would render a cell susceptible. Further steps that allow the uncoating of the viral particle at the right subcellular localization have been intensively studied. These “entry” steps could determine cell permissiveness and often define tissue and host tropism. Therefore applying the right and, when possible, straightforward experimental approaches would shorten avenues to the complete knowledge of this first and key step of any viral life cycle. Mammarenaviruses are enveloped viruses that enter the host cell via receptor-mediated endocytosis. In this chapter we present a set of customized experimental approaches and tools that were used to describe the entry of Junín virus (JUNV), and other New World mammarenavirus members, into mammalian cells.

Key words

  • Virus
  • Receptor recognition
  • Endocytosis pathways

This is a preview of subscription content, access via your institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-4939-6981-4_8
  • Chapter length: 21 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   139.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-6981-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   179.99
Price excludes VAT (USA)
Hardcover Book
USD   179.99
Price excludes VAT (USA)
Fig. 1

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Cordo SM, Cesio y AM, Candurra NA (2005) Polarized entry and release of Junín virus, a new world arenavirus. J Gen Virol 86:1475–1479

    CAS  CrossRef  PubMed  Google Scholar 

  2. Martinez MG, Cordo SM, Candurra NA (2008) Involvement of cytoskeleton in Junín virus entry. Virus Res 138:17–25

    CAS  CrossRef  PubMed  Google Scholar 

  3. Martinez MG, Cordo SM, Candurra NA (2007) Characterization of Junín arenavirus cell entry. J Gen Virol 88:1776–1784

    CAS  CrossRef  PubMed  Google Scholar 

  4. Martinez MG, Forlenza MB, Candurra NA (2009) Involvement of cellular proteins in Junín arenavirus entry. Biotechnol J 4:866–870

    CAS  CrossRef  Google Scholar 

  5. Contigiani MS, Sabattini MS (1977) Virulencia diferencial de cepas de virus Junín por marcadores biológicos en ratones y cobayos. Medicina (B Aires) 37:244–251

    Google Scholar 

  6. Martinez MG, Bialecki MA, Belouzard S, Cordo SM, Candurra NA, Whittaker GR (2013) Utilization of human DC-SIGN and L-SIGN for entry and infection of host cells by the new world arenavirus, Junín virus. Biochem Biophys Res Commun 441:612–617

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  7. Damonte EB, Mersich SE, Candurra NA (1994) Intracellular processing and transport of Junín virus glycoproteins influences virion infectivity. Virus Res 34:317–326

    CAS  CrossRef  PubMed  Google Scholar 

  8. Sanchez A, Pifat D, Kenyon RH, Peters CJ, McCormick JB, Kiley MP (1989) Junín virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses. J Gen Virol 70:1125–1132

    CAS  CrossRef  PubMed  Google Scholar 

  9. Damonte EB, Mersich SE, Candurra NA, Coto CE (1986) Cross reactivity between Junín and Tacaribe viruses as determined by neutralization test and immunoprecipitation. Med Microbiol Immunol 175:85–88

    CAS  CrossRef  PubMed  Google Scholar 

  10. Castilla V, Mersich SE, Damonte EB (1991) Lysosomotropic compounds inhibiting the multiplication of Junín virus. Rev Argent Microbiol 23:86–89

    CAS  PubMed  Google Scholar 

  11. Benmerah A, Bayrou M, Cerf-Bensussan N, Dautry-Varsat A (1992) Inhibition of clathrin- coated pit assembly by an EPS15 mutant. J Cell Sci 112:1303–1311

    Google Scholar 

  12. Damke H, Baba T, Warnock DE, Schmid SL (1994) Induction of mutant dynamin specifically blocks endocytic coated vesicle formation. J Cell Biol 127:915–934

    CAS  CrossRef  PubMed  Google Scholar 

  13. Li G, Stahl PD (1993) Structure-function relationship of the small GTPase rab5. J Biol Chem 15:24475–24480

    Google Scholar 

  14. Negre D, Mangeot PE, Duisit G, Blanchard S, Vidalain PO, Leissner P, Winter AJ, Rabourdin-Combe C, Mehtali M, Moullier P, Darlix JL, Cosset FL (2000) Characterization of novel safe lentiviral vectors derived from simian immunodeficiency virus (SIVmac251) that efficiently transduce mature human dendritic cells. Gene Ther 7:1613–1623

    CAS  CrossRef  PubMed  Google Scholar 

  15. Robertson MJ, Deane FM, Stahlschmidt W, von Kleist L, Haucke V, Robinson PJ, McCluskey A (2014) Synthesis of the Pitstop family of clathrin inhibitors. Nat Protoc 9:1592–1606

    CAS  CrossRef  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Sandra M. Cordo .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2018 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Martínez, M.G., Forlenza, M.B., Candurra, N.A., Cordo, S.M. (2018). Entry Studies of New World Arenaviruses. In: Salvato, M. (eds) Hemorrhagic Fever Viruses. Methods in Molecular Biology, vol 1604. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6981-4_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6981-4_8

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6980-7

  • Online ISBN: 978-1-4939-6981-4

  • eBook Packages: Springer Protocols