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An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses

Part of the Methods in Molecular Biology book series (MIMB,volume 1604)

Abstract

An important aspect of virological surveillance is the identification of the detected viruses. Broad surveillance, that typically employs deep sequencing of collected tissue samples, provides the investigator with many sequence files constructed from overlapping stretches of DNA sequences. Directed surveillance for viruses of a specific taxonomic group provides the investigator with sequence files from cDNA amplified using specific primers to conserved viral regions. Here we will describe general approaches to identify hemorrhagic viral agents through phylogenetic analysis of cDNA sequences obtained during surveillance activities.

Key words

  • Hemorrhagic viruses
  • Phylogenetics
  • Databases
  • Alignment
  • Genetic trees
  • Bioinformatics

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Correspondence to Juan David Rodas Ph.D. .

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Díaz, F.J., Paternina, L.E., Rodas, J.D. (2018). An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses. In: Salvato, M. (eds) Hemorrhagic Fever Viruses. Methods in Molecular Biology, vol 1604. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6981-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6981-4_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6980-7

  • Online ISBN: 978-1-4939-6981-4

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