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Murine Models for Viral Hemorrhagic Fever

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Hemorrhagic Fever Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1604))

Abstract

Hemorrhagic fever (HF) viruses, such as Lassa, Ebola, and dengue viruses, represent major human health risks due to their highly contagious nature, the severity of the clinical manifestations induced, the lack of vaccines, and the very limited therapeutic options currently available. Appropriate animal models are obviously critical to study disease pathogenesis and develop efficient therapies. We recently reported that the clone 13 (Cl13) variant of the lymphocytic choriomeningitis virus (LCMV-Cl13), a prototype arenavirus closely related to Lassa virus, causes in some mouse strains endothelial damage, vascular leakage, platelet loss, and death, mimicking pathological aspects typically observed in Lassa and other HF syndromes. This model has the advantage that the mice used are fully immunocompetent, allowing studies on the contribution of the immune response to disease progression. Moreover, LCMV is very well characterized and exhibits limited pathogenicity in humans, allowing handling in convenient BSL-2 facilities. In this chapter we outline protocols for the induction and analysis of arenavirus-mediated pathogenesis in the NZB/LCMV model, including mouse infection, virus titer determination, platelet counting, phenotypic analysis of virus-specific T cells, and assessment of vascular permeability.

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Authors and Affiliations

  1. Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, 92037, USA

    Rosana Gonzalez-Quintial & Roberto Baccala

Authors
  1. Rosana Gonzalez-Quintial
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  2. Roberto Baccala
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Correspondence to Roberto Baccala .

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Editors and Affiliations

  1. School of Medicine, University of Maryland, Baltimore, Maryland, USA

    Maria S. Salvato

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Gonzalez-Quintial, R., Baccala, R. (2018). Murine Models for Viral Hemorrhagic Fever. In: Salvato, M. (eds) Hemorrhagic Fever Viruses. Methods in Molecular Biology, vol 1604. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6981-4_20

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  • DOI: https://doi.org/10.1007/978-1-4939-6981-4_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6980-7

  • Online ISBN: 978-1-4939-6981-4

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