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Functional Viability: Measurement of Synaptic Vesicle Pool Sizes

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Book cover Cell Viability Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1601))

Abstract

Neurons and their function of conveying information across a chemical synapse are highly regulated systems. Impacts on their functional viability can occur independently from changes in morphology. Here we describe a method to assess the size of synaptic vesicle pools using live cell fluorescence imaging and a genetically encoded probe (pHluorin). Assessing functional parameters such as the size of synaptic vesicle pools can be a valuable addition to common assays of neuronal cell viability as they demonstrate that key cellular functions are intact.

The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6960-9_23

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Acknowledgments

This work was supported by Else-Kröner-Fresenius Stiftung grant 2012_A35.

J.K.W. performed this work in fulfillment of the requirements for obtaining the degree “Dr. rer. biol. hum.” at the University of Erlangen-Nuremberg.

Supplementary Files

Exemplary MATLAB code for processing a vesicle pool size recording can be found in the GitHub repository available at https://github.com/janawrosch/VesiclePoolSizes.

Author information

Authors and Affiliations

  1. Department of Psychiatry and Psychotherapy, Friedrich-Alexander University of Erlangen-Nuremberg, Schwabachanlage 6, 91054, Erlangen, Germany

    Jana K. Wrosch & Teja W. Groemer

Authors
  1. Jana K. Wrosch
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  2. Teja W. Groemer
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Corresponding author

Correspondence to Jana K. Wrosch .

Editor information

Editors and Affiliations

  1. Friedrich-Alexander University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Erlangen, Germany

    Daniel F. Gilbert

  2. Friedrich-Alexander University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Erlangen, Germany

    Oliver Friedrich

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Wrosch, J.K., Groemer, T.W. (2017). Functional Viability: Measurement of Synaptic Vesicle Pool Sizes. In: Gilbert, D., Friedrich, O. (eds) Cell Viability Assays. Methods in Molecular Biology, vol 1601. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6960-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6960-9_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6959-3

  • Online ISBN: 978-1-4939-6960-9

  • eBook Packages: Springer Protocols

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