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Endotoxin Removal from Escherichia coli Bacterial Lysate Using a Biphasic Liquid System

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Book cover Microbial Toxins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1600))

Abstract

Lipopolysaccharide (LPS, endotoxin, pyrogen) which is a component of the outer membrane of most Gram-negative bacteria is a troubling contaminant of crude bacteriophage suspension. Therefore, its removal is important for bacteriophage applications especially in preparations dedicated for use in therapy with bacterial infections treatment. The method presented here is used for extractive removal of endotoxins from bacteriophage preparations with a water immiscible solvent such as 1-octanol. During extraction most of the phage lytic activity is retained in the aqueous phase, while endotoxin accumulates in the organic solvent. The levels of endotoxin in the aqueous bacteriophage rich fraction are determine by Limulus Amebocyte Lysate or EndoLISA assay and are extremely low.

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Authors and Affiliations

  1. Laboratory of Biomedical Chemistry – “Neolek”, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Rudolfa Weigla 12, Wroclaw, PL-53-114, Poland

    Janusz Boratyński & Bożena Szermer-Olearnik

Authors
  1. Janusz Boratyński
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  2. Bożena Szermer-Olearnik
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Corresponding author

Correspondence to Janusz Boratyński .

Editor information

Editors and Affiliations

  1. Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Schleswig-Holstein, Germany

    Otto Holst

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Boratyński, J., Szermer-Olearnik, B. (2017). Endotoxin Removal from Escherichia coli Bacterial Lysate Using a Biphasic Liquid System. In: Holst, O. (eds) Microbial Toxins. Methods in Molecular Biology, vol 1600. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6958-6_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6958-6_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6956-2

  • Online ISBN: 978-1-4939-6958-6

  • eBook Packages: Springer Protocols

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