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Organ Regeneration pp 85–95Cite as

Generation of Functional Thyroid Tissue Using 3D-Based Culture of Embryonic Stem Cells

Part of the Methods in Molecular Biology book series (MIMB,volume 1597)

Abstract

During the last decade three-dimensional (3D) cultures of pluripotent stem cells have been intensively used to understand morphogenesis and molecular signaling important for the embryonic development of many tissues. In addition, pluripotent stem cells have been shown to be a valid tool for the in vitro modeling of several congenital or chronic human diseases, opening new possibilities to study their physiopathology without using animal models. Even more interestingly, 3D culture has proved to be a powerful and versatile tool to successfully generate functional tissues ex vivo. Using similar approaches, we here describe a protocol for the generation of functional thyroid tissue using mouse embryonic stem cells and give all the details and references for its characterization and analysis both in vitro and in vivo. This model is a valid approach to study the expression and the function of genes involved in the correct morphogenesis of thyroid gland, to elucidate the mechanisms of production and secretion of thyroid hormones and to test anti-thyroid drugs.

Key words

  • Thyroid development
  • Embryonic stem cells
  • Regenerative medicine
  • Hypothyroidism

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  • DOI: 10.1007/978-1-4939-6949-4_7
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Author notes

    Authors and Affiliations

    1. Institute of Interdisciplinary Research in Molecular Human Biology (IRIBHM), Université Libre de Bruxelles, 808 route de Lennik, 1070, Brussels, Belgium

      Francesco Antonica, Dominika Figini Kasprzyk, Andrea Alex Schiavo, Mírian Romitti & Sabine Costagliola

    2. Department of Physiology, Development and Neuroscience, University of Cambridge, Downing street, Cambridge, CB2 3EG, UK

      Francesco Antonica

    Authors
    1. Francesco Antonica
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    2. Dominika Figini Kasprzyk
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    3. Andrea Alex Schiavo
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    4. Mírian Romitti
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    5. Sabine Costagliola
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    Corresponding author

    Correspondence to Sabine Costagliola .

    Editor information

    Editors and Affiliations

    1. Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan

      Takashi Tsuji

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    Antonica, F., Kasprzyk, D.F., Schiavo, A.A., Romitti, M., Costagliola, S. (2017). Generation of Functional Thyroid Tissue Using 3D-Based Culture of Embryonic Stem Cells. In: Tsuji, T. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1597. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6949-4_7

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    • DOI: https://doi.org/10.1007/978-1-4939-6949-4_7

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-6947-0

    • Online ISBN: 978-1-4939-6949-4

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