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Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture

Part of the Methods in Molecular Biology book series (MIMB,volume 1597)

Abstract

A three-dimensional (3D) tissue generated in vitro is a promising source to study developmental biology and regenerative medicine. In the last decade, Yoshiki Sasai’s group have developed a 3D stem cell culture technique known as SFEBq and demonstrated that embryonic stem cells (ESCs) have an ability to self-organize stratified neural tissue including 3D-retina. Furthermore, we have reported that ESC-derived retinal tissue can form an optic cup and a ciliary margin, which are unique structures in the developing retina. In this review, we focus on self-organizing culture technique to generate 3D-retina from human ESCs.

Key words

  • SFEBq culture
  • Human ESCs
  • Retina
  • Neural retina
  • RPE
  • Optic cup
  • Ciliary margin

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  • DOI: 10.1007/978-1-4939-6949-4_2
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Acknowledgments

This article is dedicated to late Dr. Yoshiki Sasai, a great mentor and gifted scientist who pioneered this field. A.K. is grateful to Drs. Koichi Saito, Toru Kimura, and the members of Sasai Lab and Eiraku Lab for fruitful discussion.

Competing financial interests

A.K. is employed by Sumitomo Dainippon Pharma Co., Ltd. T.N. is employed by Sumitomo Chemical Co., Ltd. The authors are inventors on patent applications.

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Correspondence to Atsushi Kuwahara or Mototsugu Eiraku .

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Kuwahara, A., Nakano, T., Eiraku, M. (2017). Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture. In: Tsuji, T. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1597. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6949-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6949-4_2

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  • Publisher Name: Humana Press, New York, NY

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