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Organ Regeneration pp 1–16Cite as

Generation of Various Telencephalic Regions from Human Embryonic Stem Cells in Three-Dimensional Culture

Part of the Methods in Molecular Biology book series (MIMB,volume 1597)

Abstract

In the developing embryo, telencephalon arises from the rostral portion of the neural tube. The telencephalon further subdivides into distinct brain regions along the dorsal-ventral (DV) axis by exogenous patterning signals. Here, we describe a protocol for in vitro generation of various telencephalic regions from human embryonic stem cells (ESCs). Dissociated human ESCs are reaggregated in a low-cell-adhesion 96-well plate and cultured as floating aggregates. Telencephalic neural progenitors are efficiently generated when ESC aggregates are cultured in serum-free medium containing TGFβ inhibitor and Wnt inhibitor. In long-term culture, the telencephalic neural progenitors acquire cortical identities and self-organize a stratified cortical structure as seen in human fetal cortex. By treatment with Shh signal, the telencephalic progenitors acquire ventral (subpallial) identities and generate lateral ganglionic eminence (LGE) and medial ganglionic eminence (MGE). In contrast, by treatment with Wnt and BMP signals, their regional identities shift to more dorsal side that generates choroid plexus and medial palllium (hippocampal primordium).

Key words

  • SFEBq culture
  • Human ESCs
  • Telencephalon
  • Cerebral cortex
  • Ganglionic eminence
  • Medial pallium
  • Hippocampus

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  • DOI: 10.1007/978-1-4939-6949-4_1
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References

  1. Wilson SW, Houart C (2004) Early steps in the development of the forebrain. Dev Cell 6(2):167–181

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  2. Wonders CP, Anderson SA (2006) The origin and specification of cortical interneurons. Nat Rev Neurosci 7(9):687–696

    CAS  CrossRef  PubMed  Google Scholar 

  3. Monuki ES, Porter FD, Walsh CA (2001) Patterning of the dorsal telencephalon and cerebral cortex by a roof plate-Lhx2 pathway. Neuron 32(4):591–604

    CAS  CrossRef  PubMed  Google Scholar 

  4. Lehtinen MK, Bjornsson CS, Dymecki SM et al (2013) The choroid plexus and cerebrospinal fluid: emerging roles in development, disease, and therapy. J Neurosci 33(45):17553–17559. doi:10.1523/JNEUROSCI.3258-13.2013

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  5. Bystron I, Blakemore C, Rakic P (2008) Development of the human cerebral cortex: Boulder Committee revisited. Nat Rev Neurosci 9(2):110–122. doi:10.1038/nrn2252

    CAS  CrossRef  PubMed  Google Scholar 

  6. Tole S, Christian C, Grove EA (1997) Early specification and autonomous development of cortical fields in the mouse hippocampus. Development 124(24):4959–4970

    CAS  PubMed  Google Scholar 

  7. Sousa VH, Fishell G (2010) Sonic hedgehog functions through dynamic changes in temporal competence in the developing forebrain. Curr Opin Genet Dev 20(4):391–399. doi:10.1016/j.gde.2010.04.008

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  8. Sigurdsson T, Duvarci S (2015) Hippocampal-prefrontal interactions in cognition, behavior and psychiatric disease. Front Syst Neurosci 9:190. doi:10.3389/fnsys.2015.00190

    PubMed  Google Scholar 

  9. Eiraku M, Sasai Y (2012) Self-formation of layered neural structures in three-dimensional culture of ES cells. Curr Opin Neurobiol 22(5):768–777. doi:10.1016/j.conb.2012.02.005

    CAS  CrossRef  PubMed  Google Scholar 

  10. Sasai Y (2013) Next-generation regenerative medicine: organogenesis from stem cells in 3D culture. Cell Stem Cell 12(5):520–530. doi:10.1016/j.stem.2013.04.009

    CAS  CrossRef  PubMed  Google Scholar 

  11. Sasai Y (2013) Cytosystems dynamics in self-organization of tissue architecture. Nature 493(7432):318–326. doi:10.1038/nature11859

    CAS  CrossRef  PubMed  Google Scholar 

  12. Eiraku M, Watanabe K, Matsuo-Takasaki M et al (2008) Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals. Cell Stem Cell 3(5):519–532. doi:10.1016/j.stem.2008.09.002

    CAS  CrossRef  PubMed  Google Scholar 

  13. Watanabe M, Kang YJ, Davies LM et al (2012) BMP4 sufficiency to induce choroid plexus epithelial fate from embryonic stem cell-derived neuroepithelial progenitors. J Neurosci 32(45):15934–15945. doi:10.1523/JNEUROSCI.3227-12.2012

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  14. Kadoshima T, Sakaguchi H, Nakano T et al (2013) Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc Natl Acad Sci U S A 110:20284–20289. doi:10.1073/pnas.1315710110

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  15. Lancaster MA, Renner M, Martin CA et al (2013) Cerebral organoids model human brain development and microcephaly. Nature 501(7467):373–379. doi:10.1038/nature12517

    CAS  CrossRef  PubMed  Google Scholar 

  16. Sakaguchi H, Kadoshima T, Soen M et al (2015) Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue. Nat Commun 6:8896. doi:10.1038/ncomms9896

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  17. Watanabe K, Kamiya D, Nishiyama A et al (2005) Directed differentiation of telencephalic precursors from embryonic stem cells. Nat Neurosci 8:288–296

    CAS  CrossRef  PubMed  Google Scholar 

  18. Watanabe K, Ueno M, Kamiya D et al (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25:681–686

    CAS  CrossRef  PubMed  Google Scholar 

  19. Wataya T, Ando S, Muguruma K et al (2008) Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation. Proc Natl Acad Sci U S A 105(33):11796–11801. doi:10.1073/pnas.0803078105

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  20. Danjo T, Eiraku M, Muguruma K et al (2011) Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals. J Neurosci 31(5):1919–1933. doi:10.1523/JNEUROSCI.5128-10.2011

    CAS  CrossRef  PubMed  Google Scholar 

  21. Nasu M, Takata N, Danjo T et al (2012) Robust formation and maintenance of continuous stratified cortical neuroepithelium by laminincontaining matrix in mouse ES cell culture. PLoS One 7:e53024. doi:10.1371/journal.pone.0053024

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  22. Levine AJ, Brivanlou AH (2007) Proposal of a model of mammalian neural induction. Dev Biol 308(2):247–256

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  23. Fuccillo M, Rallu M, McMahon AP et al (2004) Temporal requirement for hedgehog signaling in ventral telencephalic patterning. Development 131(20):5031–5040

    CAS  CrossRef  PubMed  Google Scholar 

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Author notes

    Authors and Affiliations

    1. Cell Asymmetry team, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan

      Taisuke Kadoshima

    2. Asubio Pharma Co., Ltd., Kobe, Hyogo, Japan

      Taisuke Kadoshima

    3. In Vitro Histogenesis team, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan

      Hideya Sakaguchi & Mototsugu Eiraku

    4. Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan

      Hideya Sakaguchi

    Authors
    1. Taisuke Kadoshima
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    2. Hideya Sakaguchi
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    3. Mototsugu Eiraku
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    Editor information

    Editors and Affiliations

    1. Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan

      Takashi Tsuji

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    Kadoshima, T., Sakaguchi, H., Eiraku, M. (2017). Generation of Various Telencephalic Regions from Human Embryonic Stem Cells in Three-Dimensional Culture. In: Tsuji, T. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1597. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6949-4_1

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    • DOI: https://doi.org/10.1007/978-1-4939-6949-4_1

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-6947-0

    • Online ISBN: 978-1-4939-6949-4

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