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In Vivo Model of Small Intestine

Part of the Methods in Molecular Biology book series (MIMB,volume 1597)

Abstract

The utilization of human pluripotent stem cells (hPSCs) offers new avenues in the generation of organs and opportunities to understand development and diseases. The hPSC-derived human intestinal organoids (HIOs) provide a new tool to gain insights in small intestinal development, physiology, and associated diseases. Herein, we provide a method for orthotropic transplantation of HIOs in immunocompromised mice. This method highlights the specific steps to successful engraftment and provides insight into the study of bioengineered human small intestine.

Key words

  • Bioengineered small intestine
  • Human intestinal organoids
  • Pluripotent stem cells
  • Transplantation
  • In vivo model
  • Mesentery
  • Kidney capsule

The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6949-4_18

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6949-4_18

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Correspondence to Michael A. Helmrath .

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Mahe, M.M., Brown, N.E., Poling, H.M., Helmrath, M.A. (2017). In Vivo Model of Small Intestine. In: Tsuji, T. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1597. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6949-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6949-4_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6947-0

  • Online ISBN: 978-1-4939-6949-4

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