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Lipidomics pp 61-79 | Cite as

Ion-Mobility Mass Spectrometry for Lipidomics Applications

  • Giuseppe Paglia
  • Bindesh Shrestha
  • Giuseppe AstaritaEmail author
Protocol
Part of the Neuromethods book series (NM, volume 125)

Abstract

Among lipidomics’ major challenges, there is the molecular complexity of the lipidome. State-of-the-art technology, such as ion mobility (IM) spectrometry, is a promising new tool for supporting lipidomics research. IM is a gas-phase electrophoretic technique that enables the separation of ions in the gas phase according to their charge, shape, and size. IM separation, which occurs on a time scale of milliseconds, is compatible with modern mass spectrometers with microsecond scan speeds. Thus, IM-mass spectrometry (MS) can be integrated into conventional lipidomics MS workflows to improve separation and enhance peak capacity, spectral clarity, and fragmentation specificity. Furthermore, IM allows for the determination of the collision cross section (CCS), an orthogonal physicochemical measure that can be used, together with accurate mass and fragmentation information, to increase the confidence of lipid identification. In recent years, the expanding sophistication of hardware and software products has enabled IM-MS to perform an increasingly important role in traditional lipidomic approaches. In this chapter, we present IM-MS procedures for lipidomics research.

Key words

IM Lipid Collision cross section Drift time Travelling-wave IM 

Notes

Acknowledgments

We would like to thank Drs. David Grant, Andrea Armirotti, Will Thompson, Michal Kliman, Hans Vissers, Kevin Giles, Jonathan Williams, Nick Tomczyk, and Suraj Dhungana for discussions we found most enlightening.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Giuseppe Paglia
    • 1
  • Bindesh Shrestha
    • 2
  • Giuseppe Astarita
    • 2
    • 3
    Email author
  1. 1.European Academy of Bolzano/BozenCenter for BiomedicineBolzanoItaly
  2. 2.Waters CorporationMilfordUSA
  3. 3.Department of Biochemistry and Molecular & Cellular BiologyGeorgetown UniversityWashingtonUSA

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