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Construction of Protein Switches by Domain Insertion and Directed Evolution

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Synthetic Protein Switches

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1596))

Abstract

A protein switch is a protein that changes between inactive (“off”) and active (“on”) states in response to a biomolecule or physical signal. These switches can be created by fusing two domains in such a way that the activity of the output domain is regulated by the input domain’s recognition of an input signal (such as the binding of a molecule, recognition of light). Here, we describe several methods for randomly fusing two domains to create domain insertion libraries from which protein switches can be identified by selections and/or screens.

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Author information

Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N. Charles St, Baltimore, MD, 21218, USA

    Lucas F. Ribeiro, Tiana D. Warren & Marc Ostermeier

Authors
  1. Lucas F. Ribeiro
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  2. Tiana D. Warren
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  3. Marc Ostermeier
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Corresponding author

Correspondence to Marc Ostermeier .

Editor information

Editors and Affiliations

  1. Fachbereich Biologie, Technische Universität Darmstadt, Darmstadt, Germany

    Viktor Stein

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Ribeiro, L.F., Warren, T.D., Ostermeier, M. (2017). Construction of Protein Switches by Domain Insertion and Directed Evolution. In: Stein, V. (eds) Synthetic Protein Switches. Methods in Molecular Biology, vol 1596. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6940-1_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6940-1_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6938-8

  • Online ISBN: 978-1-4939-6940-1

  • eBook Packages: Springer Protocols

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