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T-Cell Trafficking pp 59–71Cite as

Visualizing Endogenous Effector T Cell Egress from the Lymph Nodes

Part of the Methods in Molecular Biology book series (MIMB,volume 1591)

Abstract

Local anatomy of lymphoid tissues during infection has emerged as a critical regulator of immunity; thus, studying the cellular choreography in the context of an intact tissue environment in situ is crucial. Following an infection, the local pathogen-specific T cell migration and the subsequent egress of effector T cells from the draining lymph nodes are important and complex biological processes. The mechanisms that regulate this complex process can now be investigated by directly visualizing T cell dynamics in vivo using intravital two-photon (2P) microscopy. In addition, static whole-mount imaging technique can provide us with a comprehensive assessment of global changes in the distribution of cellular populations within an intact tissue. Thus, in this chapter, we detail methods to visualize the migration and egress of endogenous antigen-specific CD8 T cells following viral infection using two methods—intravital 2P microscopy and multicolor whole-mount in situ tetramer staining.

Keywords

  • T cell egress
  • Sphingosine-1-phosphate receptor
  • Intravital microscopy
  • In situ tetramer staining

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  • DOI: 10.1007/978-1-4939-6931-9_5
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Author information

Authors and Affiliations

  1. Department of Immunology, University of Connecticut Health, 263 Farmington Ave, Farmington, CT, 06030, USA

    Manisha Menon & Kamal M. Khanna

  2. Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Via Olgettina 58, Milan, 20132, Italy

    Alexandre P. Benechet

Authors
  1. Manisha Menon
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  2. Alexandre P. Benechet
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  3. Kamal M. Khanna
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Corresponding author

Correspondence to Kamal M. Khanna .

Editor information

Editors and Affiliations

  1. Institute of Cardiovascular Sciences, University of Birmingham, Birmingham, United Kingdom

    George Edward Rainger

  2. Institute of Inflammation and Ageing, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

    Helen M. Mcgettrick

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Menon, M., Benechet, A.P., Khanna, K.M. (2017). Visualizing Endogenous Effector T Cell Egress from the Lymph Nodes. In: Rainger, G., Mcgettrick, H. (eds) T-Cell Trafficking. Methods in Molecular Biology, vol 1591. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6931-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6931-9_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6929-6

  • Online ISBN: 978-1-4939-6931-9

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