Food Allergens pp 147-161 | Cite as

Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy

  • Eman Ali Ali
  • Ryosuke Nakamura
  • Franco H. Falcone
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1592)

Abstract

Allergen-specific Immunoglobulin E (IgE) determination lies at the heart of diagnosis of sensitization to food and other allergens. In the past few years, reporter systems capable of detecting the presence of allergen-specific IgE have been developed by several labs. These rely on humanized rat basophil leukemia cell lines stably transfected with reporter genes such as firefly luciferase. In this chapter, we describe protocols for the use of the RS-ATL8 cell line (IgE cross-linking-induced luciferase expression; EXiLE) in 96-well and 384-well formats. We also describe optional treatment steps for enveloped virus and complement inactivation.

Key words

Reporter system Luciferase Humanized RBL IgE Allergy RS-ATL8 EXiLE 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Eman Ali Ali
    • 1
  • Ryosuke Nakamura
    • 2
  • Franco H. Falcone
    • 1
  1. 1.Division of Molecular Therapeutics and Formulation, School of PharmacyUniversity of NottinghamNottinghamUK
  2. 2.Division of Medicinal Safety ScienceNational Institute of Health SciencesSetagaya-kuJapan

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