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Fluorescent Probes for the Analysis of Labile Metals in Brain Cells

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Book cover Metals in the Brain

Part of the book series: Neuromethods ((NM,volume 124))

Abstract

The complex biological roles of transition metals create a challenge for investigating how cells of the brain interact with each other in healthy functioning and disease states. When imaging metal ions in such cells, many factors of the cellular environment need to be considered, especially when using a wide range of imaging agents to enable reliable interpretation of images and results. In this chapter, we focus on the use of fluorescent sensors for metals in a neuronal cellular environment, beginning with a summary of available fluorophores currently in the market, as well as some suggestions toward finding more tailored sensors for specific questions. We then describe the features of fluorescent probes, and highlight the parameters most crucial to different types of biological investigations. Finally, we provide a detailed method to prepare, treat, and image cellular models that should be applicable to a diverse range of fluorescent sensors, with suggestions for methods to analyze results using image analysis software.

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Authors and Affiliations

  1. School of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia

    Jacek L. Kolanowski, Clara Shen & Elizabeth J. New

Authors
  1. Jacek L. Kolanowski
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  2. Clara Shen
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  3. Elizabeth J. New
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Corresponding author

Correspondence to Elizabeth J. New .

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Editors and Affiliations

  1. Department of Pathology, University of Melbourne, Melbourne, Victoria, Australia

    Anthony R. White

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Kolanowski, J.L., Shen, C., New, E.J. (2017). Fluorescent Probes for the Analysis of Labile Metals in Brain Cells. In: White, A. (eds) Metals in the Brain. Neuromethods, vol 124. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6918-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6918-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6916-6

  • Online ISBN: 978-1-4939-6918-0

  • eBook Packages: Springer Protocols

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