Bacterial Detection Using Peptide-Based Platform and Impedance Spectroscopy
Antimicrobial peptides have the ability to function as bio-recognition elements in the detection of bacteria. For instance, we showed that Leucocin A, an antimicrobial peptide from class IIa bacteriocins, binds gram-positive Listeria monocytogenes with higher affinity than other gram-positive bacteria like S. aureus, L. innocua, and E. faecalis. The binding was detected using impedance spectroscopy when Leucocin A immobilized on impedance electrodes binds bacteria from a sample. Here we highlight the strength of utilizing Leucocin A as a bio-recognition probe in biosensor platforms and provide details on its application in real-time bacterial detection using electrochemical impedance spectroscopy. A simple new generation impedance array analyzer is utilized that works at very low frequencies to identify interactions between peptide and the target bacteria.
Key wordsPeptide-based biosensors Antimicrobial peptide Leucocin A Impedance spectroscopy Bacterial detection Listeria monocytogenes
We acknowledge the Natural Sciences and Engineering Research Council of Canada (NSERC) for supporting this research. We also thank CanBiocin Edmonton Inc. for providing all strains of bacteria. H. Etayash is the recipient of Alberta Innovates Technology Future Scholarship.
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