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Filtration and Analysis of Circulating Cancer Associated Cells from the Blood of Cancer Patients

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Book cover Biosensors and Biodetection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1572))

Abstract

Filtration is one of the most efficient methods to remove red and white blood cells from whole blood, while retaining larger cells on the surface of the filter. Precision pore microfilters, such as the CellSieve™ microfilters, are ideally suited for this purpose, as they are strong, with uniform pore size and distribution, and have low fluorescent background required for microscopic image analysis. We present a system to implement the filtration of whole blood in combination with CellSieve™ microfilters that is simple and straightforward to use. Being that the blood of cancer patients often contains both tumor cells and stromal cells associated with cancer that are larger than normal blood cells, microfiltration shows great promise in better understanding these cell types. Accurate identification and characterization of cancer associated cells has led to increased specificity as it relates to CTCs and epithelial–mesenchymal transition cells (EMTs) and enabled the identification of previously unknown cell types, such as cancer associated macrophage-like cells (CAMLs). Using a system that isolates both CTCs and circulating stromal cells, clinicians can better diagnose cancer patients to determine therapy, monitor treatment, and watch for recurrence.

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Acknowledgments

This research is funded by the U.S. Army Research Office (ARO) and the Defense Advanced Research Projects Agency (DARPA) (W911NF-14-C-0098). The content of the information does not necessarily reflect the position or the policy of the US Government.

Author information

Authors and Affiliations

  1. Creatv MicroTech, Inc., 11609 Lake Potomac Drive, Potomac, MD, 20854, USA

    Cha-Mei Tang & Platte T. Amstutz

  2. Creatv MicroTech, Inc., 9900 Belward Campus Drive, Suite 330, Rockville, MD, 20850, USA

    Peixuan Zhu & Shuhong Li

  3. Creatv MicroTech, Inc., 2242 W. Harrison Street, Suite 109B, Chicago, IL, 60612, USA

    Olga V. Makarova

  4. Creatv MicroTech, Inc., 1 Deer Park Drive, Suite L-4, Monmouth, Junction, NJ, 08852, USA

    Daniel L. Adams

Authors
  1. Cha-Mei Tang
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  2. Peixuan Zhu
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  3. Shuhong Li
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  4. Olga V. Makarova
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  5. Platte T. Amstutz
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  6. Daniel L. Adams
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Corresponding author

Correspondence to Cha-Mei Tang .

Editor information

Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Ben Prickril

  2. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Avraham Rasooly

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Tang, CM., Zhu, P., Li, S., Makarova, O.V., Amstutz, P.T., Adams, D.L. (2017). Filtration and Analysis of Circulating Cancer Associated Cells from the Blood of Cancer Patients. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_32

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  • DOI: https://doi.org/10.1007/978-1-4939-6911-1_32

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6910-4

  • Online ISBN: 978-1-4939-6911-1

  • eBook Packages: Springer Protocols

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