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Colorimetric Detection of Acetyl Xylan Esterase Activities

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Book cover Protein-Carbohydrate Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1588))

Abstract

Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates. Whereas assays using pNP-acetate, α-naphthyl acetate, and 4-methylumbelliferyl acetate (4MUA) are emphasized, common methods used to measure AcXE activity towards carbohydrate analogs (e.g., acetylated p-nitrophenyl β-d-xylopyranosides) and various acetylated xylans are also described. Strengths and limitations of the colorimetric assays are highlighted.

The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6899-2_23

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6899-2_23

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Acknowledgments

This work was supported by a grant to G.M. from Ella and Georg Ehrnrooth foundation, Finland and an ERC Consolidator Grant to E.M. (BHIVE—648925). We thank Professor M. Tenkanen for her critical review of the manuscript.

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Authors and Affiliations

  1. Department of Bioproducts and Biosystems, Aalto University, 00076, Kemistintie 1, Espoo, Aalto, Finland

    Galina Mai-Gisondi

  2. Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, M5S 3E5, Canada

    Emma R. Master

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  1. Galina Mai-Gisondi
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  2. Emma R. Master
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Correspondence to Emma R. Master .

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Editors and Affiliations

  1. Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada

    D. Wade Abbott

  2. Biotechnology Institute, University of Groningen, Groningen, The Netherlands

    Alicia Lammerts van Bueren

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Mai-Gisondi, G., Master, E.R. (2017). Colorimetric Detection of Acetyl Xylan Esterase Activities. In: Abbott, D., Lammerts van Bueren, A. (eds) Protein-Carbohydrate Interactions. Methods in Molecular Biology, vol 1588. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6899-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6899-2_5

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