Erratum to: Colorimetric Detection of Acetyl Xylan Esterase Activities

Erratum
Part of the Methods in Molecular Biology book series (MIMB, volume 1588)

Tables 1 and 2 in Chapter 5 were originally published with incorrect molecular drawings. These have been corrected and the chapter references have been updated.

Table 1

Summary of reported methods for reaction termination and product detection in assays containing pNP-acetate , α-naphthyl acetate , and 4MUA

Substrate

Developing reagent

Product detection

Reference (s)

p-Nitrophenyl acetate

(pNP-acetate)

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None

p-nitrophenol at 410 nm

[5, 9–12]

None

p-nitrophenol at 420 nm

[6, 13–16]

None

p-nitrophenol at 405 nm

[17–22]

Sodium carbonate (Na2CO3)

p-nitrophenol at 405 nm

[23]

none

Acetic acid detection kit from Boehringer Mannheim

[8]

α-naphthyl acetate

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Fast Garnet GBC in sodium dodecyl sulfate; incubate at room temperature for 15 min

α-naphthol in complex with developing reagent at 560 nm

[16–18, 24]

Fast Corinth V salt in sodium acetate buffer (pH 4.3) containing Tween 20; incubate at room temperature for 10 min

α-naphthol in complex with developing reagent at 535 nm

[25–27]

none

α-naphthol directly at 321 nm

[28]

4-methylumbelliferyl acetate (4MUA)

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Citric acid to decrease pH to 2–3

4-methylumbelliferone (4-MU) at 354 nm

[7]

Table 2

Summary of noncommercial , synthesized carbohydrate analogs used to detect AcXE activity

Substrate

Reported approach to product detection

Reference(s)

Acetylated methyl β-d-xylopyranosides (e.g., 2,3,4-tri-O-acetylated methyl-β-xylopyranoside)

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Acetylated xylobiose

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• Gas-liquid chromatography (GLC-MS)

• Acetic acid detection with K-ACETRM acetic acid kit from Megazyme

• TLC detection of released sugars

1H NMR determination of regioselectivity

[2, 9, 29–31]

Deoxy and fluoro derivatives of methyl β-d-xylopyranoside diacetates (e.g., 2-deoxy-2-fluoro-3,4-diacetylated methyl β-d-xylopyranoside)

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• TLC detection of released sugars

[32, 33]

Monoacetylated p-nitrophenyl β-d-xylopyranosides (e.g., 2-O-acetyl nitrophenyl β-d-xylopyranoside)

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Addition of Na2B4O7 (or N2CO3) followed by p-nitrophenol detection at 405 nm

[34–36]

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Bioproducts and BiosystemsAalto UniversityEspoo, AaltoFinland
  2. 2.Department of Chemical Engineering and Applied ChemistryUniversity of TorontoTorontoCanada

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