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Probing the Complex Architecture of Multimodular Carbohydrate-Active Enzymes Using a Combination of Small Angle X-Ray Scattering and X-Ray Crystallography

  • Mirjam Czjzek
  • Elizabeth Ficko-Blean
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1588)

Abstract

The various modules in multimodular carbohydrate-active enzymes (CAZymes) may function in catalysis, carbohydrate binding, protein-protein interactions or as linkers. Here, we describe how combining the biophysical techniques of Small Angle X-ray Scattering (SAXS) and macromolecular X-ray crystallography (XRC) provides a powerful tool for examination into questions related to overall structural organization of ultra multimodular CAZymes.

Key words

Small-angle X-ray scattering SAXS X-ray Crystallography Carbohydrate-active enzymes CAZymes Carbohydrate binding module CBM Glycoside hydrolase Cohesin Dockerin Multimodular Structure Dissect and build 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.UPMC Univ Paris 06, CNRS, UMR 8227, Integrative Biology of Marine ModelsSorbonne UniversiteRoscoffFrance

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